Part:BBa_K4202000:Design

mazE(codon optimization for Bacillus subtilis)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 135
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Splitting the combined ORF in BBa_K302034

Source

Escherichia coli str. K-12 substr. MG1655, sequence found from https://www.ncbi.nlm.nih.gov/gene/947245 and The condon optimization is performed using the online tool (https://www.genscript.com.cn/gensmart-free-gene-codon-optimization.html) provided by Genescript.